Cytological and microbiological examination of bovine milk in Prototheca-infected dairy herd

In healthy milk the somatic cell count (SCC) is less than 100.000 cells per mL, although in presence of infectious agents, especially pathogenic bacteria, SCC can be extremely high. Cytological appearance inflammation mammary gland depends on origin and time-course inflammatory response. According to predominant type there suppurative, pyogranulomatous, granulomatous lymphocytic inflammation. Prototheca spp. as facultative pathogen creates chronic with high reduced production. The aim our study was elaborate a practical accurate laboratory procedure enhance precision differentiation, provide quick easy-to-perform detection for algae mastitis cases. 95 quarter samples determined by California Mastitis Test, cellular density estimation differentiation were carried out microscopically evaluation, while Prothoteca investigated help specific culture medium (PIM agar). CMT results, values showed 67.3% prevalence mastitis, from which 6.3% clinical 93.7% subclinical. Comparing results estimated cellularity indicated tight correlation. negative quarters 60% neutrophil granulocytes, positive but cases these present 74%. Interestingly only 60.25% macrophages lymphocytes 17% 18.3%, respectively. Moreover, remarkable cytological pattern notified some that spp., statistical analysis correlation between ratio (p=0.005). conclusion techniques applied seems helpful supplementary methods reveal pathological background well monitor progression reactions.